An efficient method for the rapid establishment of Epstein‐Barr virus immortalization of human B lymphocytes
Identifieur interne : 009212 ( Main/Exploration ); précédent : 009211; suivant : 009213An efficient method for the rapid establishment of Epstein‐Barr virus immortalization of human B lymphocytes
Auteurs : H. Oh ; J. Oh [Corée du Sud] ; S. Choi [Corée du Sud] ; S. Kim ; W. Han [Corée du Sud] ; T. Kim ; D. Park [Corée du Sud] ; C. Jun [Corée du Sud]Source :
- Cell Proliferation [ 0960-7722 ] ; 2003-08.
Abstract
Abstract. Several methods have been developed for the immortalization of B lymphocytes by Epstein‐Barr virus (EBV). We developed an efficient method which reduces the time from culture initiation to immortalization and cryopreservation. Two infections of EBV to lymphocytes, and the use of phorbol ester‐induced EBV stock significantly improved immortalization efficiency and reduced the time between initiation and immortalization and cryopreservation. The resulting cell bank was used to produce DNA for genetic studies focusing on the genes involved in immune and autistic disorders.
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DOI: 10.1046/j.1365-2184.2003.00276.x
Affiliations:
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Several methods have been developed for the immortalization of B lymphocytes by Epstein‐Barr virus (EBV). We developed an efficient method which reduces the time from culture initiation to immortalization and cryopreservation. Two infections of EBV to lymphocytes, and the use of phorbol ester‐induced EBV stock significantly improved immortalization efficiency and reduced the time between initiation and immortalization and cryopreservation. The resulting cell bank was used to produce DNA for genetic studies focusing on the genes involved in immune and autistic disorders.</div></front></TEI><affiliations><list><country><li>Corée du Sud</li></country></list><tree><noCountry><name sortKey="Kim, S" sort="Kim, S" uniqKey="Kim S" first="S." last="Kim">S. Kim</name><name sortKey="Kim, T" sort="Kim, T" uniqKey="Kim T" first="T." last="Kim">T. Kim</name><name sortKey="Oh, H" sort="Oh, H" uniqKey="Oh H" first="H." last="Oh">H. Oh</name></noCountry><country name="Corée du Sud"><noRegion><name sortKey="Oh, J" sort="Oh, J" uniqKey="Oh J" first="J." last="Oh">J. Oh</name></noRegion><name sortKey="Choi, S" sort="Choi, S" uniqKey="Choi S" first="S." last="Choi">S. Choi</name><name sortKey="Han, W" sort="Han, W" uniqKey="Han W" first="W." last="Han">W. Han</name><name sortKey="Jun, C" sort="Jun, C" uniqKey="Jun C" first="C." last="Jun">C. Jun</name><name sortKey="Jun, C" sort="Jun, C" uniqKey="Jun C" first="C." last="Jun">C. Jun</name><name sortKey="Park, D" sort="Park, D" uniqKey="Park D" first="D." last="Park">D. Park</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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